Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. log concentrations of samples and reference at each dilution. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. For Western blot, 293T cells in 6-well plate were transfected with SARS-CoV-2 S, SARS. Reader Documentation Version No. 40266 Email: [email protected]) at room temperature. Guidelines to using the Qubit Flourimeter. 100 μl of 2% EB (Sigma-Aldrich, St. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with a 10×. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 01–10 fmol/well (0. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. fluorescence). Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. During the room temperature incubation (step 9. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. We use the SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. We use Spectramax M5 microplate reader in our lab. Fluorescein 0. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). The plates were centrifuged at the end of incubation, and fluorescent signals in supernatants were measured using a SpectraMax™ M5 microplate reader, with excitation at 485 nm and emission at 520 nm. 0 . Description Figure 1. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Plate chamber design of the SpectraMax category 1 microplate readers. Release of free AMC by proteolytic activity was monitored on Molecular Devices M5 plate reader with excitation and emission wavelengths of 360 and 460 nm, respectively. 1% Tween-20 and bound secondary was detected using Ultra TMB (Thermo Fisher); signals were read on a Molecular Devices Spectra Max M5. Copia stocks a full line of used microplate readers. reader (Molecular Devices). 5 mmol/L Li 2 SO 4 for 48 h. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Wells with an OD 600[0. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. 9. Plate chamber design of the SpectraMax category 1 microplate readers. The Back Panel Figure 1. , 2018b). Plate Analysis 9. 4: Test tube cover. Representative fluorescence images of the cell-containing scaffolds and well plate are shown in Fig. 2. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Reader Documentation Version No. Plates were shaken continuously at medium speed, and temperature was kept at 37°C. Step 1: Open the SoftMax Pro IMAP FP protocol. Cell growth was monitored by measuring OD values at 600 nm using a SpectraMax M5 plate reader (Molecular Devices, Silicon Valley, CA, United States). Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. One unit of hexokinase is the amount of enzyme that will generate 1. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Supersedes 1. Fluorescence experiments were carried out using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). com 9. Documents. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Microplate Reader Shelf. Briefly, Nunc microtiter plates were coated overnight at 2° to 8°C with KLH (3 μg/ml; IMMUCOTHEL, biosyn Arzneimittel GmbH) prepared in PBS. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Published online 2016 Jul 13. Circular Dichroism. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Microplate Automation & Detection. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. OD 600 and luminescence were measured at 30 minute intervals for 48 hours. Multimode Reader Comparison Chart. The decrease in absorbance at 340 nm was analyzed for the 200 s of the reaction, which corresponds to the initial rate of the reaction. You can also interpret your results using integrated data analysis software. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Discovery of CRBN as a target of thalidomide: a breakthrough for progress in the development of protein degraders. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. g. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Wide range of applications The VersaMax reader covers a wide range of applications:To monitor the increase rate, fluorescence measurement is then performed at 37°C at 10-s intervals for 10 min with Ex/Em 450/510 nm in the plate reader (Spectramax M5 plate reader, Molecular Devices, For each bacterial sample, we performed the reactions in duplicate and averaged the results. Fluorescence measurements were performed on a SpectraMax M5 plate reader with an excitation of 440 nm. Third this recommendation. Microplate Readers. 3. plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). Reader Documentation Version No. Viability assays were performed in 384-well microtiter plates with biologic and technical duplicates. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. For experiments with TGX-221, the cells were treated with 200 nM of TGX-221 throughout the duration of the experiment. The plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 10 μL of each SCGal-Ura and SC-Ura culture were then transferred to 1 mL fresh SC-URA and allowed to grow to saturation overnight. Fluorescence was measured with the Spectramax M5 plate reader (Molecular Devices). A solution of phenol red-free MEM containing 1. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. 3 Kg) Regulatory Regulatory CE and TUV marked. PURPOSE. We therefore gathered salient. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected] H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Page 1 of 8 . Cell morphology HeLa & caski cells were, respectively, plated in 6-well plates to logarithmic growth phase. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The optical density (OD) at 450 nm was recorded by SpectraMax M5 plate reader (Molecular Devices, CA). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The concentration of infectious virus in both stock. After 2 h of incubation, the plates were shaken, and the absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm (OD450). The fluorescence measurements of cell experiments were conducted with a SpectraMax M5 plate reader. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Information on how to reserve the plate readerMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. We use Spectramax M5 microplate reader in our lab. Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. and 72 h using the CCK-8 protocol with absorbance readings at 450 nm measured with a SpectraMax M5 plate-reader (Molecular Devices. com 9. 303 unable to cal dark current. The IC50 was calculated using the DRC package in the R software program (v3. 0 and room temperature. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. 5. 2016 Aug 2; 7(31): 50027–50042. Windows® 8. Rating: 5. Lastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. After MMP9-antibody complex formation, substrate (20 μM for human and mouse assays, 10 uM for rat) was added and fluorescence was monitored in kinetic mode at 37°C on either a SpectraMax M2 or M5. Fluorescein 0. g. This procedure is illustrated in Fig. Supersedes 1. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 9000-0756. Reader Documentation Version No. com 10. 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 5. Wide range of applications. There is currently no charge for using this plate reader. The name is the same, but the attitude is completely different. 1. 5 cm) Weight 80 lbs (36. After 2 h of static incubation at room temperature, the OD 630 of the cell-free supernatants (A) and deionized water controls (Ar) was then measured using a plate reader (SpectraMax M5) at room. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. Information on how to reserve the plate readerSunnyvale, California -- October 5, 2017. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. 0200-7027. was measured atdetermined450 nm using SpectraMax M5 plate reader. 9. SpectraMax Injector Cartridge with SmartInject™ Technology. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Lucifer yellow was measured using an M5 plate reader (Molecular Probes) at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. ScanLater Western Blot Detection Cartridge. The degradation. 2. Typically the system can detect absorbance, luminescence, fluorescence,. Nonspecific binding was determined by competition with 1. PMC full text: Oncotarget. flexibility of monochromator-based systems. Following color development, absorbance was measured at 450nm using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA), and BDNF concentrations in samples were calculated using SoftMax Pro software. Models for In. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 5. Immunoblotting. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 25:1 effector to target cells, and the plates were incubated for an additional 3 hours. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:FlexStation® 3 Multi-Mode Microplate Reader. Bioluminescence assays showed that the engineered Luc-Cys was fully active when compared with 6 × His-NanoLuc prepared in our previous work (Zhang et al. ELISAs were carried out according to the manufacturer’s instructions for IL-1β (BD Biosciences, San Diego, CA), TNFα, MIP-1α, and MCP-1 (R&D Systems, Minneapolis, MN) and quantified using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA). Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Temperature variation ± 0. A reaction volume of 12 µL was placed into each well of 384-well clear bottom black-walled plate that was sealed with an oxygen impermeable membrane. About. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). M-5 The M-5 multitronic unit, or the M-5 computer, was an advanced multitronic computer system and prototype created by Doctor Richard Daystrom during. 4. S6. Shop now. $4,995. 2. Protocol for Qubit DNA HS Assay. RoHS Compliant. Molecular Devices. All values reported represent the mean of at least three independent experiments. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. Incubation. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. doi: 10. The ideal measured bioluminescence range is 10 3 –10 6 counts/well at a SpectraMax M5 plate reader using a white opaque 96-well plate. Therefore you need to ask a physicist. -Glo assay solution (Promega) at room temperature and luminescence measured at 1000 ms −1 integration on a SpectroMax M5. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Apoptosis was measured by annexin V–fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) labeling followed by fluorescence flow cytometry as previously described. Assay for Misfolded SOD1. Information on how to reserve the plate reader Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. This is a result of the light being attenuated and scattered by the. a SpectraMax M5 plate reader (Molecular Devices LLC) to detect the absorbance value at 450 nm. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. Certain functionalities in the table above requires specific configurations. The absorbance was measured using a SpectraMax M5 plate reader from Molecular Divices (San Jose, CA, USA). The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Major items of equipment include the following: Agilent MR400 400 MHz two-channel PFG NMR. Cell Titer Glo reagent (Promega) was then added to each well, and luminescence (RLU) was measured on a SpectraMax M5 plate reader (Molecular Devices). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Kinetic data for Omnia® assays on the SpectraMax® M5/M5e reader. Viability was evaluated using the Cell Titer Glo Assay (Promega) and luminescence was read on a SpectraMax M5 plate reader (Molecular Devices). xlsx file, with or without the averages and normalizations. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Luminescence values were measured on a SpectraMax M5 plate reader (Molecular Devices). The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 600 in a Molecular Devices SpectraMax M5 plate reader at 30˚C with shaking prior to each reading. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 1 g/ml SDS solution in 0. Thermo Scientific Microplate Reader Verification Plates are dedicated performance verification systems helping to verify and document the key performance parameters of Thermo Scientific Multiskan FC, Multiskan Sky, Luminoskan, Fluoroskan FL, and Varioskan LUX. Spend a little more and you can get an FTDI-based USB cable. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Fluorescence was measured with the SpectraMax M5 plate reader with the same excitation and emission wavelengths as the chemical denaturation assay. S. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. Select Start>Control panel>Device Manager. 9. Unless reading plates from the bottom, put plates on the purple adapter to put them closer to the reading head. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Choose the Experiments icon. MOLECULAR DEVICES VMax Kinetic Microplate Reader For Sale. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 314 low lamp level warning. 345 East 24th Street, New York, NY. Published Dec 16, 2020 | Updated Dec 16, 2020. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. 2. Relative fluorescence was quantified by dividing the fluorescence by the OD 600 reading of the culture. For SpectraMax L or LMax II, LMax II 384 - no adapter used. com 10. 마이크로플레이트 리더 (Microplate Reader) 란, 물질의 광학적 성질 (흡광, 형광, 발광) 을 이용하여 Sample 내에 있는 Target 물질 (화합물) 의 양을 측정하는 분석장비 입니다. Information on cell morphology, localization of signal, cell count, and more is obtained with Cytation 5 imaging mode. com 10. 313 reference gain check fail. Thank you!. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Also refer to this Multimode Reader Comparison Chart. M5 plate reader at 595 nm. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 4: Test tube cover. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. IL-33 concentrations in the extracts were measured with a Quantikine ELISA kit (R&D Systems, Minneapolis, Minn) and read on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif). 0 μM), and this response was consistent in both plate reader assays and on chip (Supplementary Figure 5d. HSL_EQ_007: Use and Maintenance of a 2-8°C Refrigerator in the HPV Serology Laboratory 3. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. This user guide provides safety information, warnings, cautions, tips, and instructions for operating the instrument, connecting cables, setting up the instrument, and troubleshooting. 2. Fluorescence Microplate Reader Comparison Chart. With optimized reagents, option to operate the readers. The SpectraMax ® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Add to Cart. The StakMax stacker also allows users to feed plates into the loading area manually. Most papers they cultured bacteria to OD600>0. HSL_EQ_005: Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory 3. Plate section without PathCheck applied. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. Vials were coded so that the person running the samples was blinded to the contents. 3. 130 Watts max. Empirical Phase. 5: Schematic of the back panel of a reader. The IC50 was calculated using the DRC package in the R software. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. Plate Shaking. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 3. 1. Uncontrolled Copy for Training and Reference Purposes Only . The SpectraMax M2 and M2e are versatile and reliable. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. 51 L/g). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Other features. 5. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Application Area: Multi mode plate reader. Flow cytometry. 5 mL, pH 7. harvard. 19 , 25 Human umbilical cord endothelial cells (ATCC, Manassas, VA) were grown in 24-well plates coated with. easy to use and read multiple plates very quickly. After the plate is read, data will appear in the plate. The plate was incubated at 37 °C for 30 min. 3. Cell apoptosis assay. 95. 1. 18632/oncotarget. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Then, cells were treated with TCS of different concentra-tions (10, 20 and 40 μg/mL), according to theThe luminescent signal was quantified in a SpectraMax M5 plate reader (Molecular Devices, San Jose, CA). 3. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. 4: Test tube cover. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Gemini XPS Reader optics. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax M4 reader How does the reader perform a self check?. 5 × 10 5 counts/fmol, thus the suitable enzyme range for bioluminescence measurement is 0. Kojic acid was used as a positive control. Plate Analysis 9. A SpectraMax M5 plate reader was able to continuously incubate the microtiter plate at 37°C and measure the fluorescence (λex/λex= 529/600 nm) every 5 minutes for 1. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 A standard spectrophotometer measures the absorbance of one sample at a time. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Ensure data accuracy of your reader. ". Please contact Tatiana to reserve the plate reader time: tnl1@psu. 7°C and the bottom to 36. 2. We have run the program successfully on macOS High Sierra using the built-in Terminal and Windows 10 using Anaconda Prompt, processing data from a Molecular Devices SpectraMax M5 plate reader. ZERO BIAS - scores, article reviews, protocol conditions and more. 2. S6. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Then the plate was incubated at 37 °C for 2 h. A solution for low volume applications. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. StakMax Handling Stacker Base Unit. 5" H (50. After the plate is read, data will appear in the plate section: 10. 7. 312 gain calibration failed. 0200-0729. 7°C and the bottom to 36. Last lab had ours for years and never had a problem with it. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. When the user applies a set temperature of 37°C, the top is heated to 37. Microplate Reader, Multimode. Simply unpack it, plug it in, and begin your experiments. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Mutations in the α2–α2′ and α4–α4′ dimer interfaces affect the secondary structure of the DENV2C protein. For example, microplates containing different samp les can be read using the same orChoose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. 2. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The guide also covers the software features, the specifications, and the accessories of the systems. 5: Schematic of the back panel of a reader. The average 526/470 ratio for the sample aliquots. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. edu, 814-863-3503. Plate Reader with 5 modes of detection, UV-Vis (Abs), Fluorescence (FI) , Lumi, TRF, F Polarization, with current validations, fully inspectedOpen the Gen 5 software, 2. FeCl 3, iron standards are always included (0-4 µg /ml). The plates were washed thrice with kit wash buffer; 150 µl kit substrate was then added to each well and the plate incubated 30 min at room temperature (with shaking). SpectraTest ABS2 Absorbance Validation Package performance tests. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. 0 out of 5 $ 3,799. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. Thank you!. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 5 × 10 5 counts/fmol when measured with a Spectromax M5 plate reader using white opaque 96-well plates. Features. Select Update Driver. In the M2, M5 and Plus 384, the light. Please talk to your your local Tecan representative for the right configuration which suits your application demands. Category 2 and 3 microplate readers use gradient heating as well. GraphPad Prism was used to process the data and calculate the Km and Vmax values. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. The EPOCH2 can easily develop various types of assays (wavelength scan: 200–999 nm), and its temperature control (up to 65 c) is excellent. 305 reference level saturation. Remove plate cover. You can also interpret your results using integrated data analysis software. This is a result of the light being attenuated and scattered by the. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). Detection Modes. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0.